A combined deficiency of tissue factor and PAR-4 is associated with fatal pulmonary hemorrhage in mice

Mice with a complete absence of tissue factor (TF) die during embryonic development whereas mice with low levels of TF (Low-TF mice) survive to adulthood. Low-TF mice exhibit spontaneous hemorrhage in various organs, including the lung. In contrast, mice can survive without protease-activated receptor (PAR)-4, which is the major thrombin receptor on mouse platelets. We determined the effect of combining a deficiency PAR-4 (primary hemostasis) with a deficiency in TF (secondary hemostasis) on embryonic development and survival of adult mice

The multifrequency behaviour of the recurrent nova RS Ophiuchi

This review concentrates on the multifrequency behaviour of RS Ophiuchi and in particular during its latest outburst. Confirmation of the 1945 outburst, bipolar outflows and its possible fate as a Type Ia Supernova are discussed.Comment: 5 pages, 5 figures, in The Golden Age of Cataclysmic Variables and Related Objects, F. Giovannelli & L. Sabau-Graziati (eds.), Mem. SAIt. 83 N.2 (in press

Theoretical investigation of variable infra-red emission by cosmic dust sources

Light travel time considerations are applied to the development of the infra-red flux from a cloud of dust grains surrounding a variable cosmic source of heating radiation. The properties of individual grains are explored, and the equation of radiative transfer is solved for the case of a spherically symmetric thermal source observed at a large distance. Models of specific novae are developed from the standpoint of grains existing prior to nova outburst. The situation in which a variable primary source is surrounded by a disc of dust grains is explored, as is that in which an expanding central cavity is formed by grain evaporation. In both cases qualitative comparisons are made with observations of cosmic sources of variable thermal emission from dust grains

Binding dynamics of a monomeric SSB protein to DNA : a single-molecule multi-process approach

People Programme of the European Union’s Seventh Framework Programme [REA 334496 to B.E.B.]; Leonardo da Vinci European Union Programme (to M.F.G.); Wellcome Trust [099149/Z/12/Z, 091825/Z/10/Z]. Funding for open access charge: Wellcome Trust; University of St Andrews.Single-stranded DNA binding proteins (SSBs) are ubiquitous across all organisms and are characterized by the presence of an OB (oligonucleotide/oligosaccharide/oligopeptide) binding motif to recognize single-stranded DNA (ssDNA). Despite their critical role in genome maintenance, our knowledge about SSB function is limited to proteins containing multiple OB-domains and little is known about single OB-folds interacting with ssDNA. Sulfolobus solfataricus SSB (SsoSSB) contains a single OB-fold and being the simplest representative of the SSB-family may serve as a model to understand fundamental aspects of SSB:DNA interactions. Here, we introduce a novel approach based on the competition between Förster resonance energy transfer (FRET), protein-induced fluorescence enhancement (PIFE) and quenching to dissect SsoSSB binding dynamics at single monomer resolution. We demonstrate that SsoSSB follows a monomer-by-monomer binding mechanism that involves a positive-cooperativity component between adjacent monomers. We found that SsoSSB dynamic behaviour is closer to that of Replication Protein A than to Escherichia coli SSB; a feature that might be inherited from the structural analogies of their DNA-binding domains. We hypothesize that SsoSSB has developed a balance between highdensity binding and a highly dynamic interaction with ssDNA to ensure efficient protection of the genome but still allow access to ssDNA during vital cellular processes.Publisher PDFPeer reviewe

The Radio Light Curve of the Gamma-Ray Nova in V407 Cyg: Thermal Emission from the Ionized Symbiotic Envelope, Devoured from Within by the Nova Blast

We present multi-frequency radio observations of the 2010 nova event in the symbiotic binary V407 Cygni, obtained with the Karl G. Jansky Very Large Array and spanning 1-45 GHz and 17-770 days following discovery. This nova---the first ever detected in gamma rays---shows a radio light curve dominated by the wind of the Mira giant companion, rather than the nova ejecta themselves. The radio luminosity grew as the wind became increasingly ionized by the nova outburst, and faded as the wind was violently heated from within by the nova shock. This study marks the first time that this physical mechanism has been shown to dominate the radio light curve of an astrophysical transient. We do not observe a thermal signature from the nova ejecta or synchrotron emission from the shock, due to the fact that these components were hidden behind the absorbing screen of the Mira wind. We estimate a mass loss rate for the Mira wind of Mdot_w ~ 10^-6 M_sun/yr. We also present the only radio detection of V407 Cyg before the 2010 nova, gleaned from unpublished 1993 archival VLA data, which shows that the radio luminosity of the Mira wind varies by a factor of >~20 even in quiescence. Although V407 Cyg likely hosts a massive accreting white dwarf, making it a candidate progenitor system for a Type Ia supernova, the dense and radially continuous circumbinary material surrounding V407 Cyg is inconsistent with observational constraints on the environments of most Type Ia supernovae.Comment: Resubmitted to ApJ after incorporating referee's comment

Radio studies of novae: a current status report and highlights of new results

Novae, which are the sudden visual brightening triggered by runaway thermonuclear burning on the surface of an accreting white dwarf, are fairly common and bright events. Despite their astronomical significance as nearby laboratories for the study of nuclear burning and accretion phenomena, many aspects of these common stellar explosions are observationally not well-constrained and remain poorly understood. Radio observations, modeling and interpretation can potentially play a crucial role in addressing some of these puzzling issues. In this review on radio studies of novae, we focus on the possibility of testing and improving the nova models with radio observations, and present a current status report on the progress in both the observational front and theoretical developments. We specifically address the issues of accurate estimation of ejecta mass, multi-phase and complex ejection phenomena, and the effect of a dense environment around novae. With highlights of new observational results, we illustrate how radio observations can shed light on some of these long-standing puzzles.Comment: 19 pages, 4 figures. Review article published in the Bulletin of the Astronomical Society of India (BASI) special issue on nova